Clerics delivering the political speeches on the weekly Islamic holy day are appointed by Supreme Leader Ali Khamenei and reflect prevailing sentiment within the theocracy.
Tehran's interim Friday Prayer imam, Kazem Sedighi, spoke out against talks with Washington, dismissing the idea that Iran needs to reduce sanctions to address its current economic crisis.
Sedighi's remarks were in line with Iranian hardliners who continue to criticize the proponents of reconciliation with the West.
"To think that if the United States reconciles with us, our livelihood will prosper and our needs will be met goes against reason, experience, and the Holy Quran. God has not permitted a faithful community to live under the banner of disbelief," Sedighi said.
"Those who speak of reconciliation with the United States and trample on past experiences and the blood of martyrs should know that our people have sacrificed their blood and youth," he added.
Since Iran's regional military setbacks last year and the steep fall in the value of its currency, many officials and politicians loyal to the Islamic Republic have called for negotiations with the US, citing the need to transcend an economic crisis that has impoverished tens of millions of Iranians.