Syrian said Israel attacked targets southern Syria on Monday in a frontier zone where Israel has long been concerned about the presence of Iran-backed groups.
The Israeli military declined to comment.
A Syrian foreign ministry source said Israel had committed "a new aggression in the southern region" at dawn, the Syrian state news agency SANA reported.
Israel has mounted regular strikes against what it has described as Iranian-linked targets in Syria, where Tehran-backed forces including Lebanon's Hezbollah have deployed in support of President Bashar al-Assad during Syria's war.
A pro-Assad source familiar with the incident said an Israeli drone had struck several sites including a base operated by Hezbollah in al Baath city in Quneitra province and a second target. A third missile hit a reconnaissance tower near a Syrian army base, the source said.
The Syrian Observatory for Human Rights, a UK-based organisation which reports on the war, said an Israeli aircraft had fired rockets at two positions held by Syrian government forces and their allies in Quneitra province.
The attack had caused material damage and there was no information yet about casualties, it said.
Syria was discussed last week at a meeting in Sochi between Israeli Prime Minister Naftali Bennett and Russian President Vladimir Putin, Assad's most powerful ally.
Bennett told his cabinet on Sunday the two secured "stable and good" understandings over Syria - an allusion to deconfliction efforts with Russian forces amid continued Israeli air strikes on suspected Iranian assets.
Syria affirmed its right to respond to the attacks, the foreign ministry source quoted by SANA said.